This kit contains the NucView™488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry. The bi-functional NucView™488 Caspase-3 Substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView™633 is a far-red fluorescent mitochondrial dye. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView™633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap.

Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.

See our full selection of NucView™ substrates and kits. For more information about NucView™, see the NucView flyer and reference list under the downloads tab, or see the NucView FAQs.

NucView™ enzyme substrate technology is covered by U.S. Patents.

Biotium公司是一家在荧光技术领域独树一帜的生物技术公司，公司位于美国加利福尼亚的BAY AREA------世界上首要的生物科技中心。该公司一直致力于开发和生产用于生物医学研究的荧光指示剂及其他特殊生化产品。其产品被广泛应用于细胞生物学、蛋白质组学、分子生物学、免疫学、微生物学、诊断学、生物化学、神经科学、血液流动检测及大规模药物筛选等众多领域。Biotium公司专利产品众多，包括已经被广泛使用的实时PCR定量荧光染料EvaGreen、具有划时代意义的凝胶核酸染料GelRed和GelGreen，以及最新推出的Cheetah Taq热启动酶个CF系列蛋白质标记染料等等。