ResazurinCellViabilityAssayoffersasimple,rapid,reliable,sensitive,safeandcost-effectivemeasurementofcellviability.Thiskitperformsatleastaswellasothercommercialresazurin-basedcellproliferationassaykitssuchasalamarBlue®orCellTiter-Blue®.Resazurindetectscellviabilitybyconvertingfromanonfluorescentdyetothehighlyredfluorescentdyeresorufininresponsetochemicalreductionofgrowthmediumresultingfromcellgrowth(1-3).Continuedcellgrowthmaintainsareducedenvironmentwhileinhibitionofgrowthmaintainsanoxidizedenvironment.Reductionrelatedtogrowthcausestheredoxindicatortochangefromoxidized(nonfluorescent,blue)formtoreduced(fluorescent,red)form.Thefluorescentsignalismonitoredusing530-560nmexcitationwavelengthand590nmemissionwavelength.Theabsorbanceismonitoredat570nmand600nm.Thefluorescentorcolorimetricsignalgeneratedfromtheassayisproportionaltothenumberoflivingcellsinthesample.Resazurinsolutionissuppliedready-to-usein25mL(2500assay)or100mL(10,000assay)sizes.
Features:
- Sensitive:Requiringasfewas80cellsforreproducIBLeresults
- Simple:Single-stephomogenousassay
- KineticMonitoring:Reagentsnontoxictocells,thusallowingkineticmonitoring.
alamarBlueisaregisteredtrademarkofTrekBiosystems.CellTiter-BlueisaregisteredtrademarkofPromegaCorporation.
References
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2.Kanagaraj,R.,etal.RECQhelicaseassociateswiththeC-terminalrepeatdomainofRNApolymeraseIIduringproductiveelongationphaseoftranscription.NucleicAcidsResearchdoi:10.1093/nar/gkq697(2010).
3.Gustafsson,A.,etal.TheantimicrobialpeptideLL37anditstruncatedderivativespotentiatesproinflammatorycytokineinductionbylipoteichoicacidinwholeblood.ScandanavianJ.ofClinical&Lab.Inves.DOI:10.3109/00365513.2010.521255(2010).
4.Muzumdar,R.,etal.AcuteHumaninTherapyAttenuatesMyocardialIschemiaandReperfusionInjuryinMice.Arteriosclerosis,Thrombiosis,andVascularBIOLOGyDOI:10.1161/ATVBAHA.110.205997(2010).
5.Tottey,S.,etal.Theeffectofsourceanimalageuponextracellularmatrixscaffoldproperties.Biomaterialsdoi:10.1016/j.biomaterials.2010.09.006(2010).
6.Sarker,S.,etal.Microtitreplate-basedantibacterialassayincorporatingresazurinasanindicatorofcellgrowth,anditsapplicationintheinvitroantibacterialscreeningofphytochemicals.Methods42(2):321-24(2007).
7.Petrenko,Y.,etal.TheReductionofAlamarBluebyPeripheralBloodLymphocytesandIsolatedMitochondria.DOI612.112.94:576.311.347(2005).
8.Palomino,J.,etal.ResazurinMicrotiterAssayPlate:SimpleandInexpensiveMethodforDetectionofDrugResistanceinMycobacteriumtuberculosis.AntimicrobialAgentsandChemotherapyDOI:10.1128/AAC.46.8.2720
Biotium公司是一家在荧光技术领域独树一帜的生物技术公司，公司位于美国加利福尼亚的BAY AREA------世界上首要的生物科技中心。该公司一直致力于开发和生产用于生物医学研究的荧光指示剂及其他特殊生化产品。其产品被广泛应用于细胞生物学、蛋白质组学、分子生物学、免疫学、微生物学、诊断学、生物化学、神经科学、血液流动检测及大规模药物筛选等众多领域。Biotium公司专利产品众多，包括已经被广泛使用的实时PCR定量荧光染料EvaGreen、具有划时代意义的凝胶核酸染料GelRed和GelGreen，以及最新推出的Cheetah Taq热启动酶个CF系列蛋白质标记染料等等。
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