PMA-Lite™LEDPhotolysisDeviceisspecificallydesignedforphotoactivationofpropidiummonoazide(PMA)andothersimilardyes.ItcanalsofunctionasageneralphotolysisdevicetoprovidecontinuousIlluminationto1.5-2mL-sizedvialsinacontrolledmanner.Thedevicecanholdupto18vials.MultipleLEDlightsarepositionedtoprovideevenandmaximalilluminationfromboththesidesandbottomtoallvials.Aninternalfanisusedtoreducetheheatgeneratedfromthelightstoensurenearroomtemperaturephotolysis.Thedevicehasatimerthatcanbesetfor10,15,20or30minutesofcontinuousphotolysis.Theunithas120/240Vinternalconverter.AuniversaloutletadapterisincludedforcustomersoutsideofNorthAmerica.ThePMA-Lite™LEDPhotolysisDeviceiscoveredbyawarrantyfortwoyearsfromdateofpurchase.Seepage2oftheProductionInformationSheetforwarrantydetailsandinstructionsforobtainingservice.
PMA(catalognos.40019and40013)isaphoto-reactivedyethatbindstodsDNAwithhighaffinityandcanbeusedfortheselectivedetectionofviablepathogensbyqPCR. PMAxx™(catalogno.40069)isanewandimprovedphotoreactivedyeforviABIlityPCR.LearnmoreaboutPMAandPMAxx™.
WealsooffertheGlo-Plate™Blue,aflatLEDilluminatorforviabilityPCR. Whileourstandard PMA-Lite™ LEDphotolysisdevicewasdesignedforusewithmicrocentrifugetubes,theGlo-Plate™BluedeviceallowsformoreflexIBLeassayset-ups,includingmulti-wellplatesforhigh-throughputassays.ThebaseoftheGlo-Plate™BluechambercontainsblueLEDlightstoppedwithadiffuser,allowingforevenilluminationacrosssamples.
ReceiveafreetrialsizeofPMAxx™ withpurchaseofthePMA-Lite™LEDPhotolysisDevice.
PMA-Lite™LEDPhotolysisDevice.Eachdevicecomeswithoneremovablebluetubeadapter/rack.
OtherPMA-relatedproducts:
ForviabilityPCRofgram-negativebacteriawehighlyrecommendusingournewPMAEnhancer(31038)inconjunctionwithPMA.PMAEnhancerforGramNegativeBacteriawasdesignedtoimprovePMA-mediateddiscriminationbetweenliveanddeadgram-negativebacteria.Whenasequencefromagram-negativebacteriaisamplifiedbyPCR,samplespre-treatedwithEnhancershowadecreaseinthesignalfromdeadcells,withnochangeinthesignalfromlivecells.
Biotiumalsoprovidesstrain-specificPMAReal-TimePCRBacterialViabilitykitswithvalidatedprimersfor5selectedpathogens:M.tuberculosis,S.aureus,MRSA,Salmonella,andE.coliO157:H7.Thesekitsprovideeverythingthatyouneedfortheselectivedetectionofyourfavoritespeciesoflivebacteriabyreal-timePCR.Don’tseeyourfavoritestrain?Letusknowattechsupport@biotium.com.
Real-TimePCRBacterialViabilityKits
1.Nocker,A.,Cheung,C.Y.,andKamper,A.K.(2006).Comparisonofpropidiummonoazidewithethidiummonoazidefordifferentiationoflivevs.deadbacteriabyselectiveremovalofDNAfromdeadcells.J.MicrobioMeth.67(2),310-320.
2.Front.Microbiol.(2015),doi:10.3389/fmicb.2015.00295
3. ApplMicrobiolBiotechnol(2015),doi:10.1007/s00253-015-6715-x
DownloadthefullPMAReferenceList.
Biotium公司是一家在荧光技术领域独树一帜的生物技术公司,公司位于美国加利福尼亚的BAY AREA------世界上首要的生物科技中心。该公司一直致力于开发和生产用于生物医学研究的荧光指示剂及其他特殊生化产品。其产品被广泛应用于细胞生物学、蛋白质组学、分子生物学、免疫学、微生物学、诊断学、生物化学、神经科学、血液流动检测及大规模药物筛选等众多领域。Biotium公司专利产品众多,包括已经被广泛使用的实时PCR定量荧光染料EvaGreen、具有划时代意义的凝胶核酸染料GelRed和GelGreen,以及最新推出的Cheetah Taq热启动酶个CF系列蛋白质标记染料等等。