TheAccuBlue™ HighSensitivitydsDNAQuantitationKitofferssensitiveandselectivedetectionofpurifieddsDNAsampleswithminimaleffectsfromcommoncontaminants.Theassayprovidesa withsufficientreagentstoperform1000assaysbasedona96-wellmicroplateformat.AccuBlue™HighSensitivityQuantitationSolutionalsoisavailableseparately(Catalog#31008)forcustomerswhowishtousetheirownDNAstandards.
Theassay iscompatIBLewithmicroplatereaders,spectrofluorometersandhand-heldminifluorometers.Notethatwhiletheassayisspectrallycompatiblewithqubit®fluorometersfromThermoFisher,itmaynotbecompatiblewiththehighsensitivitydsDNAprogramonallQubit®models(seeourDNAQuantitationFAQsformoreinformation).FortheQubit®werecommendourAccuGreen™HighSensitivityKit(cat.no.31066),whichwasspeciallyformulatedforthatinstrument.
Features
- Lineardetectionrangefrom0.2–100ng
- λEx/λEm485/530nm
- Saferthan PicoGreen®orQuant-iT®dsDNAdetectionkits:thehighsensitivityreagentisimpermeabletocellmembranes
Kitsizes
- The1000assaysizekitincludesAccuBlue™HighSensitivityQuantitationSolutioncomponentsandasetof8pre-dilutedcalfthymusdsDNAstandards.
- ThetrialsizekitincludesoneDNAstandardsolutionwithinstructionsforpreparingasetofstandardsbyserialdilution.
OtherDNAQuantitationKits:
| dsDNAQuantitationKit | Lineardetectionrange(dsDNAinassay) | Ex/Em(nm) | Features |
|---|---|---|---|
| AccuBlue™NextGen | 1pg*-3ng | 468/507 | Quantitationofextremelypreciousordilutesamples.OptimalforsensitiveapplicationssuchasNGSordigitalPCR.*Limitofdetectionintherangeof1pgto5pgdependingoninstrumentsensitivity. |
| AccuClear™UltraHighSensitivity | 30pg-250ng | 468/507 | Thiskitoffersgreatsensitivityandthewidestlinearrange. |
| AccuBlue™HighSensitivity | 0.2-100ng | 485/530 | QuantitationofdsDNAfrom0.2-100ng,withasafer,membraneimpermeableDNAdye. |
| AccuGreen™HighSensitivity | 0.1-100ng | 502/523 | QuantitationofDNAusingtheQubit®fluorometerfromThermoFisher. |
| AccuBlue™BroadRange | 2-2000ng | 350/460 | ForquantitationofhighconcentrationDNAinabroadlinearrangewithbluefluorescence.Assaycanbeextendedto4000ngwithminorlossoflinearity. |
AccuBlue™technologyiscoveredbygrantedU.S.andinternationalpatents.
References
1.ConservationGenetResour(2012), doi:10.1007/s12686-011-9450-3
2.Parasites&Vectors(2012),doi:10.1186/1756-3305-5-59
3.JournalofChromatographyA(2010),doi:10.1016/j.chroma.2013.01.065
4.PLoSONE(2013), doi:10.1371/journal.pone.0054743
5.JournalofChromatographyA(2013),http://dx.doi.org/doi:10.1016/j.chroma.2013.11.039
6.JournalofBiomechanics(2013),http://dx.doi.org/10.1016/j.jbiomech.2013.10.036
7.PeerJ(2013),http://dx.doi.org/10.7717/peerj.203
8.JournalofBiomechanics(2014),http://dx.doi.org/10.1016/j.jbiomech.2013.10.036
9.AnalyticalBiochemistry(2014), http://dx.doi.org/10.1016/j.ab.2014.03.005
10.J.Bacteriol.(2014),doi:10.1128/JB.01493-14
11. TheInternationalJournalofBiochemistry&CellBIOLOGy(2014),doi:10.1016/j.biocel.2014.04.026
12.BMCOralHealth(2014),doi:10.1186/1472-6831-14-157
13.ApplicationsinPlantSciences(2015),http://dx.doi.org/10.3732/apps.1400077
14.JournalofBiotechnology(2015),http://dx.doi.org/10.1016/j.jbiotec.2015.04.006
15.ConservGenet(2015),doi:10.1007/s10592-015-0784-3
16.AmJPhysiolRegulIntegrCompPhysiol(2015),doi:10.1152/ajpregu.00421.2015
17.MarineGenomics(2015),doi:10.1016/j.margen.2015.10.010
18.Druryetal.BMCGenomics(2016),doi:10.1186/s12864-016-2583-8
19.MitochondrialDNAPartBResources(2016),http://dx.doi.org/10.1080/23802359.2016.1149787
20.AntonievanLeeuwenhoek(2017),doi:10.1007/s10482-017-0925-4
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