Fast Evagreen® qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses two of our breakthrough PCR technologies, Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.

• Evagreen® dye: Evagreen® DNA-binding dye selectively binds to dsDNA via a novel “release-on-demand” mechanism that ensures low PCR inhibition and thus permits the dye to be used at a relatively high concentration for melt curve analysis signal. Because the dye is spectrally similar to FAM or SYBR® Green I, the dye is compatible with all commercial qPCR instruments. Moreover, unlike SYBR® Green I and SYBR GreenER®, Evagreen® dye is extremely stable. Finally, Evagreen® dye is nonmutagenic and noncytotoxic.

• Cheetah™ Taq: Cheetah™ Taq is a new, chemically modified hotstart DNA polymerase. Unlike Amplitaq Gold®, Cheetah™ Taq can be activated in as little as two minutes under standard hotstart condition (i.e., 94°C in pH 8-9 Tris) with high activity recovery. Also unlike antibody-based hotstart polymerases, such as Platinum® Taq, Cheetah™ Taq is completely inactive at room temperature and has intrinsically low DNA contamination. In addition, Cheetah™ Taq is generally more stable at -20°C or 4°C than AmpliTaq Gold®, ensuring that the master mix maintains its peak performance following storage at low temperature.

Fast Evagreen® qPCR Master Mix is a 2X master mix, and is supplied with a separate vial of ROX reference dye, for use with high ROX, low ROX, or no ROX instruments. See the product information sheet for more information.

EvaGreen dye and applications are covered under US patent nos. 7,803,943 and 7,776,567 and pending international patents. Cheetah Taq is covered by pending US and international patents.

Biotium公司是一家在荧光技术领域独树一帜的生物技术公司，公司位于美国加利福尼亚的BAY AREA------世界上首要的生物科技中心。该公司一直致力于开发和生产用于生物医学研究的荧光指示剂及其他特殊生化产品。其产品被广泛应用于细胞生物学、蛋白质组学、分子生物学、免疫学、微生物学、诊断学、生物化学、神经科学、血液流动检测及大规模药物筛选等众多领域。Biotium公司专利产品众多，包括已经被广泛使用的实时PCR定量荧光染料EvaGreen、具有划时代意义的凝胶核酸染料GelRed和GelGreen，以及最新推出的Cheetah Taq热启动酶个CF系列蛋白质标记染料等等。