接收精选 Biotium 产品的免费样品注册我们的时事通讯并接收来自 Biotium 的免费样品。Biotium 目前提供精选产品的免费样品。有关详细信息，请参阅表格底部的选择。每次提交一个免费样品。仅运送至美国商业地址（无住宅地址）。新实验室启动计划我们知道让您的新实验室起步可能很困难。 Biotium 的新实验室启动计划旨在显着节省成本，以支持您在这一关键时期的研究。立即申请，我们所有行业领先的产品均可享受为期 6 个月的 25% 独家折扣（设备除外）。我们还将为您联系我们的一位专家科学家，帮助您为您的研究选择最好的产品。您符合条件吗？如果您满足以下所有要求，您就有资格参加我们的新实验室启动计划您的第一个实验室正在运行并收到您的位于美国的第一个研究资助实验室开始填写下面的表格，我们团队的一名成员将及时审查并回复您的申请。让我们让您的实验室跟上发展。返回特别优惠订阅我们的电子通讯，下一个订单可享受 20% 的折扣订阅我们的电子通讯，下一个订单满 250 美元或以上可享受 20% 的折扣。单击下面的按钮订阅我们的电子通讯。提交后，您将收到一封包含此促销活动促销代码的电子邮件。立即订阅一旦订阅，您将收到一封包含促销代码的电子邮件。仅对美国客户有效。不能与其他优惠同时使用。不能用于设备。立即购买核酸凝胶染料免疫荧光显微镜 PCR 和 DNA 扩增流式细胞术试剂了解有关这些产品的更多信息查看一抗和二抗偶联物不断增加的数千种标记的一抗和二抗的集合查看核酸凝胶染料用于在凝胶中染色 DNA 的染料具有 GelRed® 和 GelGreen®，我们更安全的 EtBr 替代品查看 PCR 和 DNA 扩增荧光染料和用于 qPCR 的预混液，具有 Biotium 的 EvaGreen® DyeViewAntibody 和蛋白质标记试剂盒使用明亮、光稳定的 CF® 染料和其他标签快速方便地标记抗体查看免疫荧光显微镜查看流式细胞术试剂细胞活力染色、增殖染料、标记抗体等用于流动查看蛋白质印迹用于蛋白质封闭、标准化和荧光检测的卓越解决方案其他优惠新实验室启动计划Biotium 提供新实验室在 Biotium 开设账户的前 6 个月可享受 25% 的折扣。我们还将为您联系 Biotium 产品专家，帮助您找到您需要的所有产品。查看优惠详情返回特别优惠购买 NucSpot® 核染色剂可享受 30% 的优惠在限定时间内，从现在起到 2023 年 6 月 30 日，我们的 NucSpot® 核染色剂可享受 30% 的折扣。结账时使用促销代码 30NUCSPOT。某些限制适用。查看 NucSpot® 核染色剂产品页面 注意：不适用于 NucSpot® 活细胞核染色剂（40081-T、40081、40082-T、40082）结账时使用促销代码 30NUCSPOT 仅对美国客户有效不能与其他优惠结合使用有效至 2023 年 6 月 30 日立即购买核复染剂细胞染色剂了解有关这些产品的更多信息查看 NucSpot® 核染色剂细胞膜不可渗透的细胞核特异性复染剂。适用于固定细胞或对活培养物中的死细胞进行染色。ViewNucSpot® Far-Red，1000X，在 DMSO 中是 7-AAD 的改进替代品，在 PE-Texas Red® 通道中显示出较少的渗色荧光。通过流式细胞术选择性检测死细胞的理想选择。其他产品Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This kit performs at least as well as other commercial resazurin-based cell proliferation assay kits such as alamarBlue® or CellTiter-Blue®.

Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This kit performs at least as well as other commercial resazurin-based cell proliferation assay kits such as alamarBlue® or CellTiter-Blue®. Resazurin detects cell viability by converting from a nonfluorescent dye to the highly red fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth (1-3). Continued cell growth maintains a reduced environment while inhibition of growth maintains an oxidized environment. Reduction related to growth causes the redox indicator to change from oxidized (nonfluorescent, blue) form to reduced (fluorescent, red) form. The fluorescent signal is monitored using 530-560 nm excitation wavelength and 590 nm emission wavelength. The absorbance is monitored at 570 nm and 600 nm. The fluorescent or colorimetric signal generated from the assay is proportional to the number of living cells in the sample. Resazurin solution is supplied ready-to-use in 25 mL (2500 assay) or 100 mL (10,000 assay) sizes.

Features:

• Sensitive: Requiring as few as 80 cells for reproducible results
• Simple: Single-step homogenous assay
• Kinetic Monitoring: Reagents nontoxic to cells, thus allowing kinetic monitoring.

alamarBlue is a registered trademark of Trek Biosystems. CellTiter-Blue is a registered trademark of Promega Corporation.

References

1. Tottey, S., et al. Extracellular matrix degradation products and low oxygen conditions enhance the regenerative potential of perivascular stem cells. Tissue Engineering doi: 10.1089/ten.TEA.2010.0188 (2010).

2. Kanagaraj, R., et al. RECQ helicase associates with the C-terminal repeat domain of RNA polymerase II during productive elongation phase of transcription. Nucleic Acids Research doi:10.1093/nar/gkq697 (2010).

3. Gustafsson, A., et al. The antimicrobial peptide LL37 and its truncated derivatives potentiates proinflammatory cytokine induction by lipoteichoic acid in whole blood. Scandanavian J. of Clinical & Lab. Inves. DOI: 10.3109/00365513.2010.521255 (2010).

4. Muzumdar, R., et al. Acute Humanin Therapy Attenuates Myocardial Ischemia and Reperfusion Injury in Mice. Arteriosclerosis, Thrombiosis, and Vascular Biology DOI:10.1161/ATVBAHA.110.205997 (2010).

5. Tottey, S., et al. The effect of source animal age upon extracellular matrix scaffold properties. Biomaterials doi:10.1016/j.biomaterials.2010.09.006 (2010).

6. Sarker, S., et al. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 42(2):321-24 (2007).

7. Petrenko, Y., et al. The Reduction of Alamar Blue by Peripheral Blood Lymphocytes and Isolated Mitochondria. DOI612.112.94:576.311.347 (2005).

8. Palomino, J., et al. Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy DOI: 10.1128/AAC.46.8.2720